There are now nearly 110,000 Arabidopsis EST sequences in the EMBL database, all of which are available through AGR. The majority of these ESTs have been released since the last full release of EMBL (release 63) and are only available if you search EMBL's daily update files. Most of these sequences have a 'tissue_type' qualifier listed in their database annotation. For the 79,163 EST sequences which have this information, the tissue types are as follows:
Number of ESTs Tissue type description
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18,947 roots
15,767 above-ground organ
12,752 green siliques
10,738 seed
5,827 flower buds
2,351 rosette
2,045 seedling hypocotyl
1,647 whole seedlings of A. thaliana ecotype
1,161 liquid-cultured seedlings
1,084 cell suspension culture of ecotype columbia
1,059 inflorescence
1,006 leaves, flowering plants
990 Flower buds of A.thaliana ecotype columbia C24
707 sliced leaves of A.thaliana ecotype columbia
678 Green siliques of A.thaliana ecotype columbia
642 leaves, seedlings
346 Dry seeds of A.thaliana ecotype columbia
333 seedlings, leaf and root
325 green shoots of A.thaliana ecotype columbia
241 roots of nitrate treated liquid-cultured A.thaliana
201 inflorescence with predominately younger flower buds
105 Flowering tips of ecotype Landsberg erecta
60 cell suspension culture of A.thaliana
49 whole plant
27 Excised pieces of roots containing nematode feeding sites
20 inflorescence apex with young flowers (<~Stage 9)
17 Above-ground organ from two to six-week old plants
16 Germinating seeds
6 7 day old germinated etiolated seeds
3 immature siliques
1 shoots
Please note that these tissue-type descriptions are listed exactly as they appear in the EMBL record. So presumably 'green siliques' and 'Green siliques of A.thaliana ecotype columbia' reflect the same tissue, though they are listed separately above. Also note that of these 79,163 EST sequences, 62,569 are sequences that have been released since the last full release of the EMBL database.
Given the speed at which new EST sequences are being released to EMBL it is highly likely that by the time you read this, these figures will be out of date!
This information will be made available as part of the Arabidopsis Genome Resource (AGR). For now, the information will be posted on the AGR web page (http://ukcrop.net/agr/) and in due course the database will be rebuilt to reflect this useful information and you will be able to search EST sequences by tissue type.